The ideal candidate will be curious, open-minded, hard-working, enthusiastic, motivated, detail oriented, organized and a team player with a proactive approach to research and development. They will seek out opportunities to facilitate understanding and make significant contributions in cross-functional teams in a collegiate and collaborative environment. This position will involve 80-90 % bench work.

What you can expect on a daily basis

• Design and build plasmids for strain engineering
• Prepare and submit samples for DNA sanger sequencing and interpret the resulting data
• Prepare gDNA and RNA
• Coordinate gDNA and RNA sample submission for sequencing
• Design primers for genotyping PCRs
• Design and execute genotyping PCRs
• Analyze, consolidate, document, and communicate genotyping PCR data to the team
• Plate microbial strains and prepare for colony PCR
• Follow scientific research principles and solid documentational practices at all times in your work
• Interpretation, statistical analysis and reporting of experimental data
• Maintain a high degree of accuracy, with meticulous attention to detail
• Deliver regular progress updates to the team
• Mentor and train other team members as required

What we expect you to know

• BS or higher degree in Microbiology, Molecular Biology, Biotechnology, Microbial Genetics, or a related discipline, with 2+ years of relevant experience (preferably industrial)
• Experience with
  • Plasmid design and construction
  • Primer design
  • PCR (including colony PCR, qPCR, digital droplet PCR)
  • Preparation of gDNA and RNA
  • DNA and protein electrophoresis
  • Microbial transformation
  • Small scale growth, protein expression and purification from microbial hosts
  • Enzyme assays
• Basic bioinformatics skills (Sequence alignments, BLAST, etc.)
• Sterile working technique
• Strong organizational skills
• Excels in a collaborative environment
• Strong verbal and written communication skills
• Ability to adapt and excel in a rapidly changing start-up environment
• Ability to troubleshoot experiments with strong problem-solving record

What we would like you to know

• Experience with
  • Manipulation of filamentous fungi and yeast
  • Transformation techniques and genome editing technology in fungi and yeast
• Genomic sequence analysis and interpretation
• Strain screening
• Mutagenesis
• Enzymatic assays (ELISA )
• Knowledge of microbial physiology/biochemistry